Specialized roles for the two UDP-glucosyltransferases UGT85K2 and UGT85K3 in hydroxynitrile glucoside metabolism in Lotus japonicus
Research output: Contribution to conference › Poster › Research
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Specialized roles for the two UDP-glucosyltransferases UGT85K2 and UGT85K3 in hydroxynitrile glucoside metabolism in Lotus japonicus. / Baden, Camilla Knudsen; Bjarnholt, Nanna; Jensen, Kenneth; Robsen, Fran; Olsen, Carl Erik; Motawia, Mohammed Saddik; Møller, Birger Lindberg; Takos, Adam Matthew; Rook, Fred.
2013.Research output: Contribution to conference › Poster › Research
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T1 - Specialized roles for the two UDP-glucosyltransferases UGT85K2 and UGT85K3 in hydroxynitrile glucoside metabolism in Lotus japonicus
AU - Baden, Camilla Knudsen
AU - Bjarnholt, Nanna
AU - Jensen, Kenneth
AU - Robsen, Fran
AU - Olsen, Carl Erik
AU - Motawia, Mohammed Saddik
AU - Møller, Birger Lindberg
AU - Takos, Adam Matthew
AU - Rook, Fred
N1 - Poster presented at the Gordon Research Conference in Metabolic Engineering, Waterville valley, New Hampshire, USA
PY - 2013
Y1 - 2013
N2 - Cyanogenic glucosides are amino-acid derived plant chemical defense compounds against generalist herbivores. They are α-hydroxynitrile glucosides that are activated by specific β-glucosidases upon tissue disruption. The unstable α-hydroxynitrile will dissociate with the release of hydrogen cyanide. The legume model Lotus japonicus contains the cyanogenic glucosides linamarin and lotaustralin, and the non-cyanogenic γ- and β-hydroxynitrile glucosides rhodiocyanoside A and D, which are also thought to function as defense compounds. Glucosylation is a key-step in the biosynthesis of hydroxynitrile glucosides as it stabilizes and detoxifies these compounds, and allows for their storage. Both the UDP-glucosyltransferases UGT85K2 and UGT85K3 are able to catalyze the synthesis of linamarin and lotaustralin, but only UGT85K2 showed significant glucosylation activity for the synthesis of rhodiocyanosides in vitro. Mutants in the UGT85K2 gene, obtained by TILLING, almost lacked rhodiocyanosides and showed severe growth defects. This suggested the toxicity of the rhodiocyanoside aglycones and supports their proposed defense role. The observed specificity of these UGTs further highlights the metabolic flexibility of the hydroxynitrile glucoside based defense pathway in Lotus japonicus.
AB - Cyanogenic glucosides are amino-acid derived plant chemical defense compounds against generalist herbivores. They are α-hydroxynitrile glucosides that are activated by specific β-glucosidases upon tissue disruption. The unstable α-hydroxynitrile will dissociate with the release of hydrogen cyanide. The legume model Lotus japonicus contains the cyanogenic glucosides linamarin and lotaustralin, and the non-cyanogenic γ- and β-hydroxynitrile glucosides rhodiocyanoside A and D, which are also thought to function as defense compounds. Glucosylation is a key-step in the biosynthesis of hydroxynitrile glucosides as it stabilizes and detoxifies these compounds, and allows for their storage. Both the UDP-glucosyltransferases UGT85K2 and UGT85K3 are able to catalyze the synthesis of linamarin and lotaustralin, but only UGT85K2 showed significant glucosylation activity for the synthesis of rhodiocyanosides in vitro. Mutants in the UGT85K2 gene, obtained by TILLING, almost lacked rhodiocyanosides and showed severe growth defects. This suggested the toxicity of the rhodiocyanoside aglycones and supports their proposed defense role. The observed specificity of these UGTs further highlights the metabolic flexibility of the hydroxynitrile glucoside based defense pathway in Lotus japonicus.
KW - Faculty of Science
KW - plant biochemistry
KW - Molecular Biology
M3 - Poster
ER -
ID: 138904536