A synthetic consortium of 100 gut commensals modulates the composition and function in a colon model of the microbiome of elderly subjects
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A synthetic consortium of 100 gut commensals modulates the composition and function in a colon model of the microbiome of elderly subjects. / Perez, Marta; Ntemiri, Alexandra; Tan, Huizi; Harris, Hugh M B; Roager, Henrik Munch; Ribière, Céline; O'Toole, Paul W.
In: Gut Microbes, Vol. 13, No. 1, e1919464, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - A synthetic consortium of 100 gut commensals modulates the composition and function in a colon model of the microbiome of elderly subjects
AU - Perez, Marta
AU - Ntemiri, Alexandra
AU - Tan, Huizi
AU - Harris, Hugh M B
AU - Roager, Henrik Munch
AU - Ribière, Céline
AU - O'Toole, Paul W
N1 - CURIS 2021 NEXS 173
PY - 2021
Y1 - 2021
N2 - Administration of cultured gut isolates holds promise for modulating the altered composition and function of the microbiota in older subjects, and for promoting their health. From among 692 initial isolates, we selected 100 gut commensal strains (MCC100) based on emulating the gut microbiota of healthy subjects, and retaining strain diversity within selected species. MCC100 susceptibility to seven antibiotics was determined, and their genomes were screened for virulence factor, antimicrobial resistance and bacteriocin genes. Supplementation of healthy and frail elderly microbiota types with the MCC100 in an in vitro colon model increased alpha-diversity, raised relative abundance of taxa including Blautia luti, Bacteroides fragilis, and Sutterella wadsworthensis; and introduced taxa such as Bifidobacterium spp. Microbiota changes correlated with higher levels of branched chain amino acids, which are health-associated in elderly. The study establishes that the MCC100 consortium can modulate older subjects' microbiota composition and associated metabolome in vitro, paving the way for pre-clinical and human trials.
AB - Administration of cultured gut isolates holds promise for modulating the altered composition and function of the microbiota in older subjects, and for promoting their health. From among 692 initial isolates, we selected 100 gut commensal strains (MCC100) based on emulating the gut microbiota of healthy subjects, and retaining strain diversity within selected species. MCC100 susceptibility to seven antibiotics was determined, and their genomes were screened for virulence factor, antimicrobial resistance and bacteriocin genes. Supplementation of healthy and frail elderly microbiota types with the MCC100 in an in vitro colon model increased alpha-diversity, raised relative abundance of taxa including Blautia luti, Bacteroides fragilis, and Sutterella wadsworthensis; and introduced taxa such as Bifidobacterium spp. Microbiota changes correlated with higher levels of branched chain amino acids, which are health-associated in elderly. The study establishes that the MCC100 consortium can modulate older subjects' microbiota composition and associated metabolome in vitro, paving the way for pre-clinical and human trials.
KW - Faculty of Science
KW - Gut microbiome
KW - Synthetic microbiome
KW - Consortium
KW - Antibiotics
KW - Alpha-diversity
KW - Elderly
KW - Branched chain amino acids
KW - BCAA
U2 - 10.1080/19490976.2021.1919464
DO - 10.1080/19490976.2021.1919464
M3 - Journal article
C2 - 33993839
VL - 13
JO - Gut Microbes
JF - Gut Microbes
SN - 1949-0976
IS - 1
M1 - e1919464
ER -
ID: 269513066