Antibiotic-treated versus germ-free rodents for microbiota transplantation studies
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Antibiotic-treated versus germ-free rodents for microbiota transplantation studies. / Lundberg, Randi; Toft, Martin Fitzner; August, Benjamin; Hansen, Axel Kornerup; Hansen, Camilla Hartmann Friis.
In: Gut Microbes, Vol. 7, No. 1, 08.01.2016, p. 68-74.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Antibiotic-treated versus germ-free rodents for microbiota transplantation studies
AU - Lundberg, Randi
AU - Toft, Martin Fitzner
AU - August, Benjamin
AU - Hansen, Axel Kornerup
AU - Hansen, Camilla Hartmann Friis
PY - 2016/1/8
Y1 - 2016/1/8
N2 - We recently investigated the applicability of antibiotic-treated recipient mice for transfer of different gut microbiota profiles. With this addendum we elaborate on perspectives and limitations of using antibiotics as an alternative to germ-free (GF) technology in microbial transplantation studies, and we speculate on the housing effect. It is possible to transfer host phenotypes via fecal transplantation to antibiotic-treated animals, but problems with reproducibility, baseline values, and antibiotic resistance genes should be considered. GF animals maintained in isolators still seem to be the best controlled models for long-term microbial transplantation, but antibiotic-treated recipients are also commonly utilized. We identify a need for systematic experiments investigating the stability of microbial transplantations by addressing 1) the recipient status as either GF, antibiotic-treated or specific pathogen free and 2) different levels of protected housing systems. In addition, the developmental effect of microbes on host physiological functions should be evaluated in the different scenarios.
AB - We recently investigated the applicability of antibiotic-treated recipient mice for transfer of different gut microbiota profiles. With this addendum we elaborate on perspectives and limitations of using antibiotics as an alternative to germ-free (GF) technology in microbial transplantation studies, and we speculate on the housing effect. It is possible to transfer host phenotypes via fecal transplantation to antibiotic-treated animals, but problems with reproducibility, baseline values, and antibiotic resistance genes should be considered. GF animals maintained in isolators still seem to be the best controlled models for long-term microbial transplantation, but antibiotic-treated recipients are also commonly utilized. We identify a need for systematic experiments investigating the stability of microbial transplantations by addressing 1) the recipient status as either GF, antibiotic-treated or specific pathogen free and 2) different levels of protected housing systems. In addition, the developmental effect of microbes on host physiological functions should be evaluated in the different scenarios.
KW - Faculty of Health and Medical Sciences
KW - animal models
KW - fecal transplantation
KW - germ-free
KW - gut microbiota
KW - mouse
KW - reproducibility
U2 - 10.1080/19490976.2015.1127463
DO - 10.1080/19490976.2015.1127463
M3 - Journal article
C2 - 26744774
VL - 7
SP - 68
EP - 74
JO - Gut Microbes
JF - Gut Microbes
SN - 1949-0976
IS - 1
ER -
ID: 157251584