Characterization of microsatellite markers isolated from the American Mink (Mustela vison) genome
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Characterization of microsatellite markers isolated from the American Mink (Mustela vison) genome. / Ansari, S.; Anistoroaei, Razvan Marian; Farid, A.; Christensen, Knud; Benkel, B.F.
In: Scientifur, Vol. 31, No. 2, 2007, p. 55-58.Research output: Contribution to journal › Journal article › Research
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TY - JOUR
T1 - Characterization of microsatellite markers isolated from the American Mink (Mustela vison) genome
AU - Ansari, S.
AU - Anistoroaei, Razvan Marian
AU - Farid, A.
AU - Christensen, Knud
AU - Benkel, B.F.
PY - 2007
Y1 - 2007
N2 - Two size-selected mink genomic libraries were constructed and recombinant colonies (n=6,144) were screened with a pool of probes, containing (AAAG)8, (AAGG)8, (AGGG)8, (ATAG)8 and (AG)15 oligonucleotides in equal amounts. A total of 44 colonies were hybridized and confirmed upon replating. Sequencing of the clones revealed 35 microsatellites with unique sequences. There were 19 di- and 6 tetra nucleotides, and the remaining 10 had complex repeats. Twenty eight (80%) were polymorphic in a panel of 20 mink from two Danish Farms.
AB - Two size-selected mink genomic libraries were constructed and recombinant colonies (n=6,144) were screened with a pool of probes, containing (AAAG)8, (AAGG)8, (AGGG)8, (ATAG)8 and (AG)15 oligonucleotides in equal amounts. A total of 44 colonies were hybridized and confirmed upon replating. Sequencing of the clones revealed 35 microsatellites with unique sequences. There were 19 di- and 6 tetra nucleotides, and the remaining 10 had complex repeats. Twenty eight (80%) were polymorphic in a panel of 20 mink from two Danish Farms.
KW - Former LIFE faculty
KW - American mink
KW - microsattelite
KW - polymorphism
M3 - Journal article
VL - 31
SP - 55
EP - 58
JO - Scientifur
JF - Scientifur
SN - 0105-2403
IS - 2
ER -
ID: 8078799