FLIPR assays of intracellular calcium in GPCR drug discovery
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FLIPR assays of intracellular calcium in GPCR drug discovery. / Hansen, Kasper Bø; Bräuner-Osborne, Hans.
G protein-coupled receptors in drug discovery. ed. / Wayne R Leifert. Vol. 552 United States : Humana Press, 2009. p. 269-278 (Methods in Molecular Biology, Vol. 552).Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research
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TY - CHAP
T1 - FLIPR assays of intracellular calcium in GPCR drug discovery
AU - Hansen, Kasper Bø
AU - Bräuner-Osborne, Hans
N1 - Keywords: Intracellular calcium measurement, G protein-coupled receptors, probenecid, FLIPR, FlexStation, NOVO star. Chapter 19
PY - 2009
Y1 - 2009
N2 - Fluorescent dyes sensitive to changes in intracellular calcium have become increasingly popular in G protein-coupled receptor (GPCR) drug discovery for several reasons. First of all, the assays using the dyes are easy to perform and are of low cost compared to other assays. Second, most non-Galpha(q)-coupled GPCRs can be tweaked to modulate intracellular calcium by co-transfection with promiscuous or chimeric/mutated G proteins making the calcium assays broadly applicable in GPCR research. Third, the price of instruments capable of measuring fluorescent-based calcium indicators has become significantly less making them obtainable even for academic groups. Here, we present a protocol for measuring changes in intracellular calcium levels in living mammalian cells based on the fluorescent calcium binding dye, fluo-4.
AB - Fluorescent dyes sensitive to changes in intracellular calcium have become increasingly popular in G protein-coupled receptor (GPCR) drug discovery for several reasons. First of all, the assays using the dyes are easy to perform and are of low cost compared to other assays. Second, most non-Galpha(q)-coupled GPCRs can be tweaked to modulate intracellular calcium by co-transfection with promiscuous or chimeric/mutated G proteins making the calcium assays broadly applicable in GPCR research. Third, the price of instruments capable of measuring fluorescent-based calcium indicators has become significantly less making them obtainable even for academic groups. Here, we present a protocol for measuring changes in intracellular calcium levels in living mammalian cells based on the fluorescent calcium binding dye, fluo-4.
KW - Former Faculty of Pharmaceutical Sciences
U2 - 10.1007/978-1-60327-317-6_19
DO - 10.1007/978-1-60327-317-6_19
M3 - Book chapter
C2 - 19513656
SN - 978-1-60327-316-9
VL - 552
T3 - Methods in Molecular Biology
SP - 269
EP - 278
BT - G protein-coupled receptors in drug discovery
A2 - Leifert, Wayne R
PB - Humana Press
CY - United States
ER -
ID: 13063072