High-intensity exercise training improves basal platelet prostacyclin sensitivity and potentiates the response to dual anti-platelet therapy in postmenopausal women
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High-intensity exercise training improves basal platelet prostacyclin sensitivity and potentiates the response to dual anti-platelet therapy in postmenopausal women. / Wickham, Kate; Nørregaard, Line Boel; Slingsby, Martina Helena Lundberg; Cheung, Stephen S; Hellsten, Ylva.
In: Biomolecules, Vol. 12, No. 10, 1501, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - High-intensity exercise training improves basal platelet prostacyclin sensitivity and potentiates the response to dual anti-platelet therapy in postmenopausal women
AU - Wickham, Kate
AU - Nørregaard, Line Boel
AU - Slingsby, Martina Helena Lundberg
AU - Cheung, Stephen S
AU - Hellsten, Ylva
N1 - CURIS 2022 NEXS 257
PY - 2022
Y1 - 2022
N2 - The risk of thrombotic events dramatically increases with age and may be accelerated in women by the cessation of endogenous estrogen production at menopause. Patients at risk of thrombosis are prescribed dual anti-platelet therapy (DAPT; aspirin and a P2Y12 antagonist) and are encouraged to participate in regular physical activity, as these modalities improve nitric oxideand prostacyclin-mediated inhibition of platelet aggregation. Methods: We assessed prostacyclin sensitivity as well as basal platelet reactivity with and without in vitro DAPT before and after an 8-week high-intensity exercise training program in 13 healthy, sedentary postmenopausal women.The training intervention consisted of three 1 h sessions per week. Isolated platelets were analyzed for thromboxane A2 receptor, thromboxane A2 synthase, cyclooxygenase-1, and prostacyclin receptor protein expression. Additionally, plasma 6-keto prostaglandin F1α and thromboxane B2 levels weredetermined. Results: Exercise training made platelets more sensitive to the inhibitory effects of prostacyclin on thromboxane-, collagen-, and adenosine 5′-diphosphate (ADP)-induced aggregation, as well as thrombin-receptor activator peptide 6- and ADP-induced aggregation with DAPT. However, there was no change in protein expression from isolated platelets or plasma thromboxane B2 andprostacyclin levels following training. Conclusion: Together, these findings emphasize the importance of promoting physical activity as a tool for reducing thrombotic risk in postmenopausal women and suggest that training status should be considered when prescribing DAPT in this cohort.
AB - The risk of thrombotic events dramatically increases with age and may be accelerated in women by the cessation of endogenous estrogen production at menopause. Patients at risk of thrombosis are prescribed dual anti-platelet therapy (DAPT; aspirin and a P2Y12 antagonist) and are encouraged to participate in regular physical activity, as these modalities improve nitric oxideand prostacyclin-mediated inhibition of platelet aggregation. Methods: We assessed prostacyclin sensitivity as well as basal platelet reactivity with and without in vitro DAPT before and after an 8-week high-intensity exercise training program in 13 healthy, sedentary postmenopausal women.The training intervention consisted of three 1 h sessions per week. Isolated platelets were analyzed for thromboxane A2 receptor, thromboxane A2 synthase, cyclooxygenase-1, and prostacyclin receptor protein expression. Additionally, plasma 6-keto prostaglandin F1α and thromboxane B2 levels weredetermined. Results: Exercise training made platelets more sensitive to the inhibitory effects of prostacyclin on thromboxane-, collagen-, and adenosine 5′-diphosphate (ADP)-induced aggregation, as well as thrombin-receptor activator peptide 6- and ADP-induced aggregation with DAPT. However, there was no change in protein expression from isolated platelets or plasma thromboxane B2 andprostacyclin levels following training. Conclusion: Together, these findings emphasize the importance of promoting physical activity as a tool for reducing thrombotic risk in postmenopausal women and suggest that training status should be considered when prescribing DAPT in this cohort.
KW - Faculty of Science
KW - Dual anti-platelet therapy
KW - Exercise
KW - Menopause
KW - Platelets
KW - Coagulation
KW - Thrombosis
KW - Prostacyclin
U2 - 10.3390/biom12101501
DO - 10.3390/biom12101501
M3 - Journal article
C2 - 36291709
VL - 12
JO - Biomolecules
JF - Biomolecules
SN - 2218-273X
IS - 10
M1 - 1501
ER -
ID: 323301666