Increased expression of endothelin B receptor in static stretch exposed porcine mitral valve leaflets
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Increased expression of endothelin B receptor in static stretch exposed porcine mitral valve leaflets. / Pedersen, Lotte Gam; Zhao, J.; Yang, J.; Thomsen, Preben Dybdahl; Gregersen, H.; Hasenkam, J.M.; Smerup, M.; Pedersen, H.D.; Olsen, Lisbeth Høier.
In: Research in Veterinary Science, Vol. 82, No. 2, 2007, p. 232-238.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Increased expression of endothelin B receptor in static stretch exposed porcine mitral valve leaflets
AU - Pedersen, Lotte Gam
AU - Zhao, J.
AU - Yang, J.
AU - Thomsen, Preben Dybdahl
AU - Gregersen, H.
AU - Hasenkam, J.M.
AU - Smerup, M.
AU - Pedersen, H.D.
AU - Olsen, Lisbeth Høier
PY - 2007
Y1 - 2007
N2 - The aim of this study was to evaluate the effect of mechanical stretch on the expression of ET-1 and ETA- and ETB-receptors in porcine mitral valve leaflets.Leaflet segments from 10 porcine mitral valves were exposed to a static stretch load of 1.5 N for 3.5 h in buffer at 37oC together with matching control segments. Subsequently, the mRNA expression of ET-1, ETA-R and ETB-R was measured by real-time RT-PCR in the chordal insertion areas.The analyses showed an increased transcription of ETB-receptors in stretch-exposed leaflet segments compared to unstretched segments median 2.23 (quartiles 1.37 and 2.70) vs. median 1.56 (quartiles 1.38 and 2.17, P=0.03) whereas the mRNA expression of ETA-receptors (P=0.90) and ET-1 (P=0.51) remained unchanged.Stretch increased the expression of ETB-receptors in porcine mitral valve leaflets. The finding could lead to a better understanding of the pathogenesis of myxomatous mitral valve disease.
AB - The aim of this study was to evaluate the effect of mechanical stretch on the expression of ET-1 and ETA- and ETB-receptors in porcine mitral valve leaflets.Leaflet segments from 10 porcine mitral valves were exposed to a static stretch load of 1.5 N for 3.5 h in buffer at 37oC together with matching control segments. Subsequently, the mRNA expression of ET-1, ETA-R and ETB-R was measured by real-time RT-PCR in the chordal insertion areas.The analyses showed an increased transcription of ETB-receptors in stretch-exposed leaflet segments compared to unstretched segments median 2.23 (quartiles 1.37 and 2.70) vs. median 1.56 (quartiles 1.38 and 2.17, P=0.03) whereas the mRNA expression of ETA-receptors (P=0.90) and ET-1 (P=0.51) remained unchanged.Stretch increased the expression of ETB-receptors in porcine mitral valve leaflets. The finding could lead to a better understanding of the pathogenesis of myxomatous mitral valve disease.
KW - Former LIFE faculty
KW - Endothelin
KW - Endothelin receptors
KW - Mitral valve
KW - Real-time PCR
KW - Stress
KW - Stretch
U2 - 10.1016/j.rvsc.2006.07.009
DO - 10.1016/j.rvsc.2006.07.009
M3 - Journal article
C2 - 17011002
VL - 82
SP - 232
EP - 238
JO - Research in Veterinary Science
JF - Research in Veterinary Science
SN - 0034-5288
IS - 2
ER -
ID: 8045011