Localization of thymosin ß10 in breast cancer cells: relationship to actin cytoskeletal remodeling and cell motility
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Localization of thymosin ß10 in breast cancer cells : relationship to actin cytoskeletal remodeling and cell motility. / Mælan, A.ase Elisabeth; Rasmussen, Trine Kring; Larsson, Lars-Inge.
In: Histochemistry and Cell Biology, Vol. 127, No. 1, 2007, p. 109-113.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Localization of thymosin ß10 in breast cancer cells
T2 - relationship to actin cytoskeletal remodeling and cell motility
AU - Mælan, A.ase Elisabeth
AU - Rasmussen, Trine Kring
AU - Larsson, Lars-Inge
PY - 2007
Y1 - 2007
N2 - Beta-thymosins are polypeptides involved in the regulation of actin polymerization and thymosin ß10 and ß4 have been implicated in sequestration of monomeric (G-) actin. Additionally, experimental overexpression of thymosin ß10 has been found to result in increases in F-actin bundles as well as in cell motility and spreading. We have studied the distribution of endogenously expressed thymosin ß10 in cultured human breast cancer cell lines. Both unperturbed monolayer cultures and wound-healing models were examined using double-staining for thymosin ß10 and polymerized (F-) actin. Our findings show that thymosin ß10 is expressed in all three-cancer cell lines (SK-BR-3, MCF-7 and MDA-MB-231) studied. No or little staining was detected in confluent cells, whereas strong staining occurred in semiconfluent cells and in cells populating monolayer wounds. Importantly, the distribution of staining for thymosin ß10 was inverse of staining for F-actin. These data support a physiological role for thymosin ß10 in sequestration of G-actin as well as in cancer cell motility.
AB - Beta-thymosins are polypeptides involved in the regulation of actin polymerization and thymosin ß10 and ß4 have been implicated in sequestration of monomeric (G-) actin. Additionally, experimental overexpression of thymosin ß10 has been found to result in increases in F-actin bundles as well as in cell motility and spreading. We have studied the distribution of endogenously expressed thymosin ß10 in cultured human breast cancer cell lines. Both unperturbed monolayer cultures and wound-healing models were examined using double-staining for thymosin ß10 and polymerized (F-) actin. Our findings show that thymosin ß10 is expressed in all three-cancer cell lines (SK-BR-3, MCF-7 and MDA-MB-231) studied. No or little staining was detected in confluent cells, whereas strong staining occurred in semiconfluent cells and in cells populating monolayer wounds. Importantly, the distribution of staining for thymosin ß10 was inverse of staining for F-actin. These data support a physiological role for thymosin ß10 in sequestration of G-actin as well as in cancer cell motility.
KW - Former LIFE faculty
KW - Thymosin ß10
KW - Breast cancer
KW - F-actin
KW - G-actin
KW - Motility
KW - Cell culture
KW - Immunofluorescence
KW - Wound healing
U2 - 10.1007/s00418-006-0208-z
DO - 10.1007/s00418-006-0208-z
M3 - Journal article
C2 - 16786322
VL - 127
SP - 109
EP - 113
JO - Histochemistry and Cell Biology
JF - Histochemistry and Cell Biology
SN - 0948-6143
IS - 1
ER -
ID: 8045394