New advanced glycation end products observed in rat urine by untargeted metabolomics after feeding with heat-treated skimmed milk powder
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New advanced glycation end products observed in rat urine by untargeted metabolomics after feeding with heat-treated skimmed milk powder. / Zhou, Xiaomin; Ulaszewska, Maria M; De Gobba, Cristian; Rinnan, Åsmund; Poulsen, Malene Wibe; Chen, Jie; Mattivi, Fulvio; Hedegaard, Rikke Vingborg; Skibsted, Leif Horsfelt; Dragsted, Lars Ove.
In: Molecular Nutrition & Food Research, Vol. 65, No. 7, 2001049, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - New advanced glycation end products observed in rat urine by untargeted metabolomics after feeding with heat-treated skimmed milk powder
AU - Zhou, Xiaomin
AU - Ulaszewska, Maria M
AU - De Gobba, Cristian
AU - Rinnan, Åsmund
AU - Poulsen, Malene Wibe
AU - Chen, Jie
AU - Mattivi, Fulvio
AU - Hedegaard, Rikke Vingborg
AU - Skibsted, Leif Horsfelt
AU - Dragsted, Lars Ove
N1 - This article is protected by copyright. All rights reserved.
PY - 2021
Y1 - 2021
N2 - Scope: Milk powder is commonly consumed throughout the world. However, advanced glycation end products (AGEs) will form in milk powder during thermal processing and long-term storage. We aimed to identify such compounds with potential as new urinary biomarkers of intake of heat-treated skimmed milk powder (HSMP).Methods and results: A parallel study was performed with different dosages of HSMP as well as hydrolyzed HSMP and untreated skimmed milk powder (SMP) in 36 rats. The 24-h urine samples on day 7 or 8 were collected and profiled by untargeted UPLC-Qtof-MS metabolomics. Statistical analysis revealed 25 metabolites differentiating SMP and HSMP; nineteen of these structures were proposed as lysine- and arginine- derived AGEs, and heterocyclic compounds.Conclusion: These metabolites may potentially serve as biomarkers of food intake pending further validation to assess intakes of heat-processed dairy foods and thus help to elucidate the effects of HSMP consumption or dietary AGEs on human health.
AB - Scope: Milk powder is commonly consumed throughout the world. However, advanced glycation end products (AGEs) will form in milk powder during thermal processing and long-term storage. We aimed to identify such compounds with potential as new urinary biomarkers of intake of heat-treated skimmed milk powder (HSMP).Methods and results: A parallel study was performed with different dosages of HSMP as well as hydrolyzed HSMP and untreated skimmed milk powder (SMP) in 36 rats. The 24-h urine samples on day 7 or 8 were collected and profiled by untargeted UPLC-Qtof-MS metabolomics. Statistical analysis revealed 25 metabolites differentiating SMP and HSMP; nineteen of these structures were proposed as lysine- and arginine- derived AGEs, and heterocyclic compounds.Conclusion: These metabolites may potentially serve as biomarkers of food intake pending further validation to assess intakes of heat-processed dairy foods and thus help to elucidate the effects of HSMP consumption or dietary AGEs on human health.
KW - Faculty of Science
KW - Skimmed milk powder
KW - Metabolomics
KW - Advanced glycation end products
KW - Maillard reaction
KW - Biomarker
U2 - 10.1002/mnfr.202001049
DO - 10.1002/mnfr.202001049
M3 - Journal article
C2 - 33559951
VL - 65
JO - Molecular Nutrition & Food Research
JF - Molecular Nutrition & Food Research
SN - 1613-4125
IS - 7
M1 - 2001049
ER -
ID: 256631368