Nitrate-rich beetroot juice ingestion reduces skeletal muscle O2 uptake and blood flow during exercise in sedentary men
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Nitrate-rich beetroot juice ingestion reduces skeletal muscle O2 uptake and blood flow during exercise in sedentary men. / Nyberg, Michael; Christensen, Peter Møller; Blackwell, Jamie R; Hostrup, Morten; Jones, Andrew M; Bangsbo, Jens.
In: Journal of Physiology, Vol. 599, No. 23, 2021, p. 5203-5214.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Nitrate-rich beetroot juice ingestion reduces skeletal muscle O2 uptake and blood flow during exercise in sedentary men
AU - Nyberg, Michael
AU - Christensen, Peter Møller
AU - Blackwell, Jamie R
AU - Hostrup, Morten
AU - Jones, Andrew M
AU - Bangsbo, Jens
N1 - This article is protected by copyright. All rights reserved.
PY - 2021
Y1 - 2021
N2 - Dietary nitrate supplementation has been shown to reduce pulmonary O2 uptake during submaximal exercise and enhance exercise performance. However, the effects of nitrate supplementation on local metabolic and hemodynamic regulation in contracting human skeletal muscle remain unclear. To address this, eight healthy young male sedentary subjects were assigned in a randomized, double-blind, crossover design to receive nitrate-rich beetroot juice (NO3, 9 mmol) and placebo (PLA) 2.5 h prior to the completion of a double-step knee-extensor exercise protocol that included a transition from unloaded to moderate-intensity exercise (MOD) followed immediately by a transition to intense exercise (HIGH). Compared with PLA, NO3 increased plasma levels of nitrate and nitrite. During MOD, leg VO2 and leg blood flow (LBF) were reduced to a similar extent (∼9-15 %) in NO3. During HIGH, leg VO2 was reduced by ∼6-10 % and LBF by ∼5-9 % (did not reach significance) in NO3. Leg VO2 kinetics was markedly faster in the transition from passive to MOD compared with the transition from MOD to HIGH both in NO3 and PLA with no difference between PLA and NO3. In NO3, a reduction in nitrate and nitrite concentration was detected between arterial and venous samples. No difference in the time to exhaustion was observed between conditions. In conclusion, elevation of plasma nitrate and nitrate reduces leg skeletal muscle VO2 and blood flow during exercise. However, nitrate supplementation does not enhance muscle VO2 kinetics during exercise, nor does it improve time to exhaustion when exercising with a small muscle mass.
AB - Dietary nitrate supplementation has been shown to reduce pulmonary O2 uptake during submaximal exercise and enhance exercise performance. However, the effects of nitrate supplementation on local metabolic and hemodynamic regulation in contracting human skeletal muscle remain unclear. To address this, eight healthy young male sedentary subjects were assigned in a randomized, double-blind, crossover design to receive nitrate-rich beetroot juice (NO3, 9 mmol) and placebo (PLA) 2.5 h prior to the completion of a double-step knee-extensor exercise protocol that included a transition from unloaded to moderate-intensity exercise (MOD) followed immediately by a transition to intense exercise (HIGH). Compared with PLA, NO3 increased plasma levels of nitrate and nitrite. During MOD, leg VO2 and leg blood flow (LBF) were reduced to a similar extent (∼9-15 %) in NO3. During HIGH, leg VO2 was reduced by ∼6-10 % and LBF by ∼5-9 % (did not reach significance) in NO3. Leg VO2 kinetics was markedly faster in the transition from passive to MOD compared with the transition from MOD to HIGH both in NO3 and PLA with no difference between PLA and NO3. In NO3, a reduction in nitrate and nitrite concentration was detected between arterial and venous samples. No difference in the time to exhaustion was observed between conditions. In conclusion, elevation of plasma nitrate and nitrate reduces leg skeletal muscle VO2 and blood flow during exercise. However, nitrate supplementation does not enhance muscle VO2 kinetics during exercise, nor does it improve time to exhaustion when exercising with a small muscle mass.
KW - Faculty of Science
KW - O2 uptake kinetics
KW - Knee-extensor exercise
KW - Nitrite
U2 - 10.1113/JP281995
DO - 10.1113/JP281995
M3 - Journal article
C2 - 34587650
VL - 599
SP - 5203
EP - 5214
JO - The Journal of Physiology
JF - The Journal of Physiology
SN - 0022-3751
IS - 23
ER -
ID: 280736810