Superparamagnetic bead interactions with functionalized surfaces characterized by an immunomicroarray
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Magneto-resistive sensors capable of detecting superparamagnetic micro-/nano-sized beads are promising alternatives to standard diagnostic assays based on absorbance or fluorescence and streptavidin-functionalized beads are widely used as an integral part of these sensors. Here we have developed an immunomicroarray for systematic studies of the binding properties of 10 different micro-/nano-sized streptavidin-functionalized beads to a biotin substrate immobilized on SiO2 with or without surface modification SiO2 surface cleaning, immobilized substrate concentration and surface blocking conditions were optimized Polyethylene glycol-based surfaces with different end groups on the anchor molecule, 2,4,6-trichloro-1,3,5-triazine (TsT), were synthesized and compared with the standard (3-aminopropyl)triethoxysilane (APTS)/glutaraldehyde chemistry APTS/glutaraldehyde, directly linked TsT and bare H2O2-activated SiO2 performed better than polyethylene glycol-modified surfaces Two beads, Masterbeads and M-280 beads, were found to give superior results compared with other bead types. Antibody/ antigen interactions, Illustrated by C-reactive protein, were best performed with Masterbeads The results provide important information concerning the surface binding properties of streptavidin-functionalized beads and the immunomicroarray can be used when optimizing the performance of bead-based biosensors.
Translated title of the contribution | Superparamagnetic bead interactions with functionalized surfaces characterized by an immunomicroarray |
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Original language | English |
Journal | Acta Biomaterialia |
Volume | 6 |
Issue number | 10 |
Pages (from-to) | 3936-3946 |
Number of pages | 11 |
ISSN | 1742-7061 |
Publication status | Published - 2010 |
- Former Faculty of Pharmaceutical Sciences
Research areas
ID: 33238365