Molecular characterization of the porcine surfactant, pulmonary-associated protein C gene
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Molecular characterization of the porcine surfactant, pulmonary-associated protein C gene. / Cirera, Susanna; Hillig, Ann-Britt Nygaard; Jensen, Henrik Michael Elvang; Skovgaard, K.; Boye, M.; Fredholm, Merete.
In: Genomics, Vol. 88, No. 5, 2006, p. 659-668.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Molecular characterization of the porcine surfactant, pulmonary-associated protein C gene
AU - Cirera, Susanna
AU - Hillig, Ann-Britt Nygaard
AU - Jensen, Henrik Michael Elvang
AU - Skovgaard, K.
AU - Boye, M.
AU - Fredholm, Merete
PY - 2006
Y1 - 2006
N2 - The surfactant, pulmonary-associated protein C (SFTPC) is a peptide secreted by the alveolar type II pneumocytes of the lung. We have characterized the porcine SFTPC gene at genomic, transcriptional, and protein levels. The porcine SFTPC is a single-copy gene on pig chromosome 14. Two transcripts were found in a newborn pig lung cDNA library: a full-length clone and a clone missing exon 5. cDNA sequence comparison revealed four synonymous and two nonsynonymous substitutions and in-frame insertions at the beginning of exon 5. Comparison of the SFTPC coding region between several mammals showed high levels of conservation. Northern blot studies showed lung-specific expression of the full-length SFTPC transcript, appearing in 50-day-old fetus and increasing during lung development. Both SFTPC transcripts were detected mainly in lung by real-time RT-PCR and they were significantly down-regulated in necrotic lungs of pigs infected with Actinobacillus pleuropneumoniae. Additionally, the protein levels were also decreased or absent in the necrotic tissue.
AB - The surfactant, pulmonary-associated protein C (SFTPC) is a peptide secreted by the alveolar type II pneumocytes of the lung. We have characterized the porcine SFTPC gene at genomic, transcriptional, and protein levels. The porcine SFTPC is a single-copy gene on pig chromosome 14. Two transcripts were found in a newborn pig lung cDNA library: a full-length clone and a clone missing exon 5. cDNA sequence comparison revealed four synonymous and two nonsynonymous substitutions and in-frame insertions at the beginning of exon 5. Comparison of the SFTPC coding region between several mammals showed high levels of conservation. Northern blot studies showed lung-specific expression of the full-length SFTPC transcript, appearing in 50-day-old fetus and increasing during lung development. Both SFTPC transcripts were detected mainly in lung by real-time RT-PCR and they were significantly down-regulated in necrotic lungs of pigs infected with Actinobacillus pleuropneumoniae. Additionally, the protein levels were also decreased or absent in the necrotic tissue.
KW - Former LIFE faculty
KW - SFTPC, SNPs, gene expression, real-time RT-PCR
KW - Actinobacillus pleuropneumoniae lung infection
KW - type II cells
U2 - 10.1016/j.ygeno.2006.04.011
DO - 10.1016/j.ygeno.2006.04.011
M3 - Journal article
C2 - 16769199
VL - 88
SP - 659
EP - 668
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 5
ER -
ID: 8026436