Immunological methods for dosimetry of heterocyclic amines
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Immunological methods for dosimetry of heterocyclic amines. / Dragsted, Lars Ove; Nielsen, S E; Heitmann, B L; Grivas, S; Frandsen, H.
Toxicology - From Cells to Man. Springer, 1996. p. 259-274 (Archives of Toxicology. Supplement, Vol. 18).Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review
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TY - GEN
T1 - Immunological methods for dosimetry of heterocyclic amines
AU - Dragsted, Lars Ove
AU - Nielsen, S E
AU - Heitmann, B L
AU - Grivas, S
AU - Frandsen, H
N1 - (Ekstern)
PY - 1996
Y1 - 1996
N2 - Carcinogenic heterocyclic amines are formed by common household cooking procedures, and it is therefore of interest to evaluate exposures in the general population. High-affinity monoclonal antibodies to 2-amino-l-methyl-6-phenyl- imidazo[4,5-b]pyridine (PhIP1) were produced and a sensitive fluorescence-based ELISA assay developed with a 50% inhibition by 150 fmoles PhIP or PhlP- metabolites modified in the 2-position. PhIP antigenic material was excreted mainly from 0-24 hours after a single meal containing fried meat in two volunteers and dropped to background levels after 48 hours. Urine samples (24 hours) were collected from 265 Danish males and females aged 40-70 years. The participants were also asked to fill in a questionnaire regarding their recent intake of fried and otherwise cooked meat. Excretion of PhlP-antigenic material was observed among those who had been eating Med meat with a median excretion of 1.5μg/24 hours. A higher median excretion among all samples of about 2.3μg/24 hours was observed when acid hydrolyzed samples were analyzed. This indicates that a large proportion of conjugated metabolites are present before hydrolysis, since these metabolites are not as efficient competitors for the antibody in ELISA. The excretion of PhIP antigenic materials showed a skewed normal distribution. These observations are close to predicted exposure rates in the general population, based on food consumption figures and analyses of PhIP in fried meat samples. PhIP antigenic material was always found to be present in urine samples from individuals who reported a recent intake of fried meat. Positive samples were also found among individuals who reported no recent intakes of fried or browned meat. The possible sources of this exposure are discussed.
AB - Carcinogenic heterocyclic amines are formed by common household cooking procedures, and it is therefore of interest to evaluate exposures in the general population. High-affinity monoclonal antibodies to 2-amino-l-methyl-6-phenyl- imidazo[4,5-b]pyridine (PhIP1) were produced and a sensitive fluorescence-based ELISA assay developed with a 50% inhibition by 150 fmoles PhIP or PhlP- metabolites modified in the 2-position. PhIP antigenic material was excreted mainly from 0-24 hours after a single meal containing fried meat in two volunteers and dropped to background levels after 48 hours. Urine samples (24 hours) were collected from 265 Danish males and females aged 40-70 years. The participants were also asked to fill in a questionnaire regarding their recent intake of fried and otherwise cooked meat. Excretion of PhlP-antigenic material was observed among those who had been eating Med meat with a median excretion of 1.5μg/24 hours. A higher median excretion among all samples of about 2.3μg/24 hours was observed when acid hydrolyzed samples were analyzed. This indicates that a large proportion of conjugated metabolites are present before hydrolysis, since these metabolites are not as efficient competitors for the antibody in ELISA. The excretion of PhIP antigenic materials showed a skewed normal distribution. These observations are close to predicted exposure rates in the general population, based on food consumption figures and analyses of PhIP in fried meat samples. PhIP antigenic material was always found to be present in urine samples from individuals who reported a recent intake of fried meat. Positive samples were also found among individuals who reported no recent intakes of fried or browned meat. The possible sources of this exposure are discussed.
KW - Faculty of Science
KW - Heterocyclic amine
KW - Skewed normal distribution
KW - Recent intake
KW - Median excretion
KW - Roasted coffee bean
UR - http://www.scopus.com/inward/record.url?scp=0029686050&partnerID=8YFLogxK
U2 - 10.1007/978-3-642-61105-6_26
DO - 10.1007/978-3-642-61105-6_26
M3 - Article in proceedings
C2 - 8678802
AN - SCOPUS:0029686050
T3 - Archives of Toxicology. Supplement
SP - 259
EP - 274
BT - Toxicology - From Cells to Man
PB - Springer
ER -
ID: 254775930