New methods and reagents to improve the ferret model for human influenza infections
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- Poster
Final published version, 3.49 MB, PowerPoint presentation
The ferret has been extensively used to study human influenza infections. However, its value as a model has suffered from the limited set of reagents and methods available for this animal. We have recently tested a large number of monoclonal antibodies cross-reacting with ferret CD markers (CD8, CD9, CD14, CD18, CD25, CD29, CD32, CD44, CD61, CD71, CD79b, CD88, CD104, CD172a and CD3) and cytokines (interferon-gamma, TNF-alpha, interleukine-4 and interleukine-8) for flow cytometry , as well as polyclonal antibodies cross-reacting with ferret immunoglobulins (IgA, IgG and IgM) for ELISA. Further improvements of the model will aim at establishing a reliable RT-PCR for ferret cytokines, as well as investigating the location of influenza receptors and viral particles in the upper and lower respiratory tract via immunohistochemistry
Original language | English |
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Publication date | 2008 |
Publication status | Published - 2008 |
Event | Graduate School of Immunology 3. Annual Meeting: Interphases between adaptive and innate immunity in inflammation and autoimmunity - Helsingør, Denmark Duration: 1 Oct 2008 → 3 Oct 2008 Conference number: 3 |
Conference
Conference | Graduate School of Immunology 3. Annual Meeting: Interphases between adaptive and innate immunity in inflammation and autoimmunity |
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Number | 3 |
Country | Denmark |
City | Helsingør |
Period | 01/10/2008 → 03/10/2008 |
- Former LIFE faculty - ferret, monoclonal antibodies, CD markers, cytokines, immunoglobulins
Research areas
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