Molecular characterization of cultivated species of the genus Pachyrhizus Rich. ex DC. by AFLP markers: calling for more data
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Molecular characterization of cultivated species of the genus Pachyrhizus Rich. ex DC. by AFLP markers : calling for more data. / Santayana, Monica; Rossel, Genoveva; Núñez, Jorge; Sørensen, Marten; Delêtre, Marc; Robles, Ronald; Fernández, Victor ; Grüneberg, Wolfgang J.; Heider, Bettina.
In: Tropical Plant Biology, Vol. 7, No. 3-4, 2014, p. 121-132.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Molecular characterization of cultivated species of the genus Pachyrhizus Rich. ex DC. by AFLP markers
T2 - calling for more data
AU - Santayana, Monica
AU - Rossel, Genoveva
AU - Núñez, Jorge
AU - Sørensen, Marten
AU - Delêtre, Marc
AU - Robles, Ronald
AU - Fernández, Victor
AU - Grüneberg, Wolfgang J.
AU - Heider, Bettina
PY - 2014
Y1 - 2014
N2 - Yam beans (Pachyrhizus DC.) are legume rootcrops that have attracted scientific interest because of highcontents of starch, protein, and iron in their roots. In this study,58 accessions of three cultivated Pachyrhizus species werecharacterized by amplified fragment length polymorphism(AFLP) molecular markers in order to estimate genetic diversityand interspecific relationships. To complement molecularmarker information, individuals from each accession wereanalyzed in order to confirmploidy levels. Eight AFLP primercombinations detected 136 (68.7 %) polymorphic bands.Shannon’s diversity indices (Hs) for each species were 1.04(P. ahipa), 1.07 (P. tuberosus), and 2.42 (P. erosus), while thetotal diversity index was 2.45. Phylogenetic analysis, principalcoordinate analysis and analysis of molecular variance(FST=0.796) all showed significant species differentiation.All accessions were diploid (2n=2x=22), which is characteristicof the tribe Phaseoleae. Finally, a misclassified accessionof P. tuberosus was identified. Molecular characterization ofaccessions is necessary for efficient management of germplasmcollections.
AB - Yam beans (Pachyrhizus DC.) are legume rootcrops that have attracted scientific interest because of highcontents of starch, protein, and iron in their roots. In this study,58 accessions of three cultivated Pachyrhizus species werecharacterized by amplified fragment length polymorphism(AFLP) molecular markers in order to estimate genetic diversityand interspecific relationships. To complement molecularmarker information, individuals from each accession wereanalyzed in order to confirmploidy levels. Eight AFLP primercombinations detected 136 (68.7 %) polymorphic bands.Shannon’s diversity indices (Hs) for each species were 1.04(P. ahipa), 1.07 (P. tuberosus), and 2.42 (P. erosus), while thetotal diversity index was 2.45. Phylogenetic analysis, principalcoordinate analysis and analysis of molecular variance(FST=0.796) all showed significant species differentiation.All accessions were diploid (2n=2x=22), which is characteristicof the tribe Phaseoleae. Finally, a misclassified accessionof P. tuberosus was identified. Molecular characterization ofaccessions is necessary for efficient management of germplasmcollections.
KW - Faculty of Science
KW - Pachyrhizus . Molecular characterization . Ploidy . AFLP .American yambean . Genetic diversity
U2 - 10.1007/s12042-014-9143-7
DO - 10.1007/s12042-014-9143-7
M3 - Journal article
VL - 7
SP - 121
EP - 132
JO - Tropical Plant Biology
JF - Tropical Plant Biology
SN - 1935-9756
IS - 3-4
ER -
ID: 129964784