Asymmetric Damage Segregation Constitutes an Emergent Population-Level Stress Response
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Asymmetric Damage Segregation Constitutes an Emergent Population-Level Stress Response. / Vedel, Søren; Nunns, Harry; Košmrlj, Andrej; Semsey, Szabolcs; Trusina, Ala.
In: Cell Systems, Vol. 3, No. 2, 13.07.2016, p. 187-198.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Asymmetric Damage Segregation Constitutes an Emergent Population-Level Stress Response
AU - Vedel, Søren
AU - Nunns, Harry
AU - Košmrlj, Andrej
AU - Semsey, Szabolcs
AU - Trusina, Ala
N1 - Copyright © 2016 Elsevier Inc. All rights reserved.
PY - 2016/7/13
Y1 - 2016/7/13
N2 - Asymmetric damage segregation (ADS) is a mechanism for increasing population fitness through non-random, asymmetric partitioning of damaged macromolecules at cell division. ADS has been reported across multiple organisms, though the measured effects on fitness of individuals are often small. Here, we introduce a cell-lineage-based framework that quantifies the population-wide effects of ADS and then verify our results experimentally in E. coli under heat and antibiotic stress. Using an experimentally validated mathematical model, we find that the beneficial effect of ADS increases with stress. In effect, low-damage subpopulations divide faster and amplify within the population acting like a positive feedback loop whose strength scales with stress. Analysis of protein aggregates shows that the degree of asymmetric inheritance is damage dependent in single cells. Together our results indicate that, despite small effects in single cell, ADS exerts a strong beneficial effect on the population level and arises from the redistribution of damage within a population, through both single-cell and population-level feedback.
AB - Asymmetric damage segregation (ADS) is a mechanism for increasing population fitness through non-random, asymmetric partitioning of damaged macromolecules at cell division. ADS has been reported across multiple organisms, though the measured effects on fitness of individuals are often small. Here, we introduce a cell-lineage-based framework that quantifies the population-wide effects of ADS and then verify our results experimentally in E. coli under heat and antibiotic stress. Using an experimentally validated mathematical model, we find that the beneficial effect of ADS increases with stress. In effect, low-damage subpopulations divide faster and amplify within the population acting like a positive feedback loop whose strength scales with stress. Analysis of protein aggregates shows that the degree of asymmetric inheritance is damage dependent in single cells. Together our results indicate that, despite small effects in single cell, ADS exerts a strong beneficial effect on the population level and arises from the redistribution of damage within a population, through both single-cell and population-level feedback.
U2 - 10.1016/j.cels.2016.06.008
DO - 10.1016/j.cels.2016.06.008
M3 - Journal article
C2 - 27426983
VL - 3
SP - 187
EP - 198
JO - Cell Systems
JF - Cell Systems
SN - 2405-4712
IS - 2
ER -
ID: 164017971