Blue ghosts: a new method for isolating amber mutants defective in essential genes of Escherichia coli
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Blue ghosts: a new method for isolating amber mutants defective in essential genes of Escherichia coli. / Brown, S; Brickman, E R; Beckwith, J.
In: Journal of Bacteriology, Vol. 146, No. 1, 1981, p. 422-5.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Blue ghosts: a new method for isolating amber mutants defective in essential genes of Escherichia coli
AU - Brown, S
AU - Brickman, E R
AU - Beckwith, J
N1 - Keywords: Escherichia coli; Genes; Genetic Techniques; Mutation
PY - 1981
Y1 - 1981
N2 - We describe a technique which permits an easy screening for amber mutants defective in essential genes of Escherichia coli. Using this approach, we have isolated three amber mutants defective in the rho gene. An extension of the technique allows the detection of ochre mutants and transposon insertions in essential genes.
AB - We describe a technique which permits an easy screening for amber mutants defective in essential genes of Escherichia coli. Using this approach, we have isolated three amber mutants defective in the rho gene. An extension of the technique allows the detection of ochre mutants and transposon insertions in essential genes.
M3 - Journal article
C2 - 7012125
VL - 146
SP - 422
EP - 425
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 1
ER -
ID: 9298214