Pancreatic alpha and beta cells are globally phase-locked
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- s41467-022-31373-6
Final published version, 5.33 MB, PDF document
The Ca2+ modulated pulsatile glucagon and insulin secretions by pancreatic alpha and beta cells play a crucial role in glucose homeostasis. However, how alpha and beta cells coordinate to produce various Ca2+ oscillation patterns is still elusive. Using a microfluidic device and transgenic mice, we recorded Ca2+ signals from islet alpha and beta cells, and observed heterogeneous Ca2+ oscillation patterns intrinsic to each islet. After a brief period of glucose stimulation, alpha and beta cells' oscillations were globally phase-locked. While the activation of alpha cells displayed a fixed time delay of similar to 20 s to that of beta cells, beta cells activated with a tunable period. Moreover, islet a cell number correlated with oscillation frequency. We built a mathematical model of islet Ca2+ oscillation incorporating paracrine interactions, which quantitatively agreed with the experimental data. Our study highlights the importance of cell-cell interaction in generating stable but tunable islet oscillation patterns.
Original language | English |
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Article number | 3721 |
Journal | Nature Communications |
Volume | 13 |
Issue number | 1 |
Number of pages | 16 |
ISSN | 2041-1723 |
DOIs | |
Publication status | Published - 28 Jun 2022 |
- PULSATILE INSULIN-SECRETION, GLUCAGON-SECRETION, CA2+ OSCILLATIONS, ELECTRICAL-ACTIVITY, MOUSE ISLETS, SUSTAINED OSCILLATIONS, PLASMA-INSULIN, BASAL INSULIN, CYCLIC-AMP, GLUCOSE
Research areas
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