Filopodia rotate and coil by actively generating twist in their actin shaft
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Filopodia rotate and coil by actively generating twist in their actin shaft. / Leijnse, Natascha; Barooji, Younes Farhangi; Arastoo, Mohammad Reza; Sonder, Stine Lauritzen; Verhagen, Bram; Wullkopf, Lena; Erler, Janine Terra; Semsey, Szabolcs; Nylandsted, Jesper; Oddershede, Lene Broeng; Doostmohammadi, Amin; Bendix, Poul Martin.
In: Nature Communications, Vol. 13, No. 1, 1636, 28.03.2022.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Filopodia rotate and coil by actively generating twist in their actin shaft
AU - Leijnse, Natascha
AU - Barooji, Younes Farhangi
AU - Arastoo, Mohammad Reza
AU - Sonder, Stine Lauritzen
AU - Verhagen, Bram
AU - Wullkopf, Lena
AU - Erler, Janine Terra
AU - Semsey, Szabolcs
AU - Nylandsted, Jesper
AU - Oddershede, Lene Broeng
AU - Doostmohammadi, Amin
AU - Bendix, Poul Martin
PY - 2022/3/28
Y1 - 2022/3/28
N2 - The authors show how tubular surface structures in all cell types, have the ability to twist and perform rotary sweeping motion to explore the extracellular environment. This has implications for migration, sensing and cell communication.Filopodia are actin-rich structures, present on the surface of eukaryotic cells. These structures play a pivotal role by allowing cells to explore their environment, generate mechanical forces or perform chemical signaling. Their complex dynamics includes buckling, pulling, length and shape changes. We show that filopodia additionally explore their 3D extracellular space by combining growth and shrinking with axial twisting and buckling. Importantly, the actin core inside filopodia performs a twisting or spinning motion which is observed for a range of cell types spanning from earliest development to highly differentiated tissue cells. Non-equilibrium physical modeling of actin and myosin confirm that twist is an emergent phenomenon of active filaments confined in a narrow channel which is supported by measured traction forces and helical buckles that can be ascribed to accumulation of sufficient twist. These results lead us to conclude that activity induced twisting of the actin shaft is a general mechanism underlying fundamental functions of filopodia.
AB - The authors show how tubular surface structures in all cell types, have the ability to twist and perform rotary sweeping motion to explore the extracellular environment. This has implications for migration, sensing and cell communication.Filopodia are actin-rich structures, present on the surface of eukaryotic cells. These structures play a pivotal role by allowing cells to explore their environment, generate mechanical forces or perform chemical signaling. Their complex dynamics includes buckling, pulling, length and shape changes. We show that filopodia additionally explore their 3D extracellular space by combining growth and shrinking with axial twisting and buckling. Importantly, the actin core inside filopodia performs a twisting or spinning motion which is observed for a range of cell types spanning from earliest development to highly differentiated tissue cells. Non-equilibrium physical modeling of actin and myosin confirm that twist is an emergent phenomenon of active filaments confined in a narrow channel which is supported by measured traction forces and helical buckles that can be ascribed to accumulation of sufficient twist. These results lead us to conclude that activity induced twisting of the actin shaft is a general mechanism underlying fundamental functions of filopodia.
KW - EMBRYONIC STEM-CELLS
KW - GROUND-STATE
KW - DYNAMICS
KW - CYTOSKELETON
KW - ADHESION
KW - FASCIN
KW - INVADOPODIA
KW - RETRACTION
KW - MAINTAINS
KW - MIGRATION
U2 - 10.1038/s41467-022-28961-x
DO - 10.1038/s41467-022-28961-x
M3 - Journal article
C2 - 35347113
VL - 13
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 1636
ER -
ID: 303445738