Label-free optical interferometric microscopy to characterize morphodynamics in living plants
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Label-free optical interferometric microscopy to characterize morphodynamics in living plants. / Ebrahimi, Samira; Moreno-Pescador, Guillermo; Persson, Staffan; Jauffred, Liselotte; Bendix, Poul Martin.
In: Frontiers in Plant Science, Vol. 14, 1156478, 2023.Research output: Contribution to journal › Review › Research › peer-review
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TY - JOUR
T1 - Label-free optical interferometric microscopy to characterize morphodynamics in living plants
AU - Ebrahimi, Samira
AU - Moreno-Pescador, Guillermo
AU - Persson, Staffan
AU - Jauffred, Liselotte
AU - Bendix, Poul Martin
PY - 2023
Y1 - 2023
N2 - During the last century, fluorescence microscopy has played a pivotal role in a range of scientific discoveries. The success of fluorescence microscopy has prevailed despite several shortcomings like measurement time, photobleaching, temporal resolution, and specific sample preparation. To bypass these obstacles, label-free interferometric methods have been developed. Interferometry exploits the full wavefront information of laser light after interaction with biological material to yield interference patterns that contain information about structure and activity. Here, we review recent studies in interferometric imaging of plant cells and tissues, using techniques such as biospeckle imaging, optical coherence tomography, and digital holography. These methods enable quantification of cell morphology and dynamic intracellular measurements over extended periods of time. Recent investigations have showcased the potential of interferometric techniques for precise identification of seed viability and germination, plant diseases, plant growth and cell texture, intracellular activity and cytoplasmic transport. We envision that further developments of these label-free approaches, will allow for high-resolution, dynamic imaging of plants and their organelles, ranging in scales from sub-cellular to tissue and from milliseconds to hours.
AB - During the last century, fluorescence microscopy has played a pivotal role in a range of scientific discoveries. The success of fluorescence microscopy has prevailed despite several shortcomings like measurement time, photobleaching, temporal resolution, and specific sample preparation. To bypass these obstacles, label-free interferometric methods have been developed. Interferometry exploits the full wavefront information of laser light after interaction with biological material to yield interference patterns that contain information about structure and activity. Here, we review recent studies in interferometric imaging of plant cells and tissues, using techniques such as biospeckle imaging, optical coherence tomography, and digital holography. These methods enable quantification of cell morphology and dynamic intracellular measurements over extended periods of time. Recent investigations have showcased the potential of interferometric techniques for precise identification of seed viability and germination, plant diseases, plant growth and cell texture, intracellular activity and cytoplasmic transport. We envision that further developments of these label-free approaches, will allow for high-resolution, dynamic imaging of plants and their organelles, ranging in scales from sub-cellular to tissue and from milliseconds to hours.
KW - digital holographic cell imaging
KW - optical coherence tomography
KW - label-free microscopy
KW - material transport
KW - intereferometric imaging
KW - plant cells and tissues
KW - speckle imaging
KW - plant morphodynamics
KW - DIGITAL HOLOGRAPHIC MICROSCOPY
KW - COHERENCE TOMOGRAPHY
KW - BIOSPECKLE ACTIVITY
KW - ROOT-GROWTH
KW - REVEALS
KW - DIFFRACTION
KW - DYNAMICS
KW - LEAF
KW - TOOL
U2 - 10.3389/fpls.2023.1156478
DO - 10.3389/fpls.2023.1156478
M3 - Review
C2 - 37284726
VL - 14
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
SN - 1664-462X
M1 - 1156478
ER -
ID: 355086543