Protocol to assess metabolic activity of Pseudomonas aeruginosa by measuring heat flow using isothermal calorimetry

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Protocol to assess metabolic activity of Pseudomonas aeruginosa by measuring heat flow using isothermal calorimetry. / Beilharz, Katrin; Kragh, Kasper Nørskov; Fritz, Blaine; Kirkegaard, Julius B.; Tolker-Nielsen, Tim; Bjarnsholt, Thomas; Lichtenberg, Mads.

In: STAR Protocols, Vol. 4, No. 2, 102269, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Beilharz, K, Kragh, KN, Fritz, B, Kirkegaard, JB, Tolker-Nielsen, T, Bjarnsholt, T & Lichtenberg, M 2023, 'Protocol to assess metabolic activity of Pseudomonas aeruginosa by measuring heat flow using isothermal calorimetry', STAR Protocols, vol. 4, no. 2, 102269. https://doi.org/10.1016/j.xpro.2023.102269

APA

Beilharz, K., Kragh, K. N., Fritz, B., Kirkegaard, J. B., Tolker-Nielsen, T., Bjarnsholt, T., & Lichtenberg, M. (2023). Protocol to assess metabolic activity of Pseudomonas aeruginosa by measuring heat flow using isothermal calorimetry. STAR Protocols, 4(2), [102269]. https://doi.org/10.1016/j.xpro.2023.102269

Vancouver

Beilharz K, Kragh KN, Fritz B, Kirkegaard JB, Tolker-Nielsen T, Bjarnsholt T et al. Protocol to assess metabolic activity of Pseudomonas aeruginosa by measuring heat flow using isothermal calorimetry. STAR Protocols. 2023;4(2). 102269. https://doi.org/10.1016/j.xpro.2023.102269

Author

Beilharz, Katrin ; Kragh, Kasper Nørskov ; Fritz, Blaine ; Kirkegaard, Julius B. ; Tolker-Nielsen, Tim ; Bjarnsholt, Thomas ; Lichtenberg, Mads. / Protocol to assess metabolic activity of Pseudomonas aeruginosa by measuring heat flow using isothermal calorimetry. In: STAR Protocols. 2023 ; Vol. 4, No. 2.

Bibtex

@article{bb683884c902488f8d334e7681f82049,
title = "Protocol to assess metabolic activity of Pseudomonas aeruginosa by measuring heat flow using isothermal calorimetry",
abstract = "Here, we present a protocol for assessing metabolic activity of bacterial populations by measuring heat flow using isothermal calorimetry. We outline the steps for preparing the different growth models of Pseudomonas aeruginosa and performing continuous metabolic activity measurements in the calScreener. We detail simple principal component analysis to differentiate between metabolic states of different populations and probabilistic logistic classification to assess resemblance to wild-type bacteria. This protocol for fine-scale metabolic measurement can aid in understanding microbial physiology. For complete details on the use and execution of this protocol, please refer to Lichtenberg et al. (2022).1",
keywords = "Cell-based Assays, Metabolism, Microbiology",
author = "Katrin Beilharz and Kragh, {Kasper N{\o}rskov} and Blaine Fritz and Kirkegaard, {Julius B.} and Tim Tolker-Nielsen and Thomas Bjarnsholt and Mads Lichtenberg",
note = "Publisher Copyright: {\textcopyright} 2023 The Authors",
year = "2023",
doi = "10.1016/j.xpro.2023.102269",
language = "English",
volume = "4",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",

}

RIS

TY - JOUR

T1 - Protocol to assess metabolic activity of Pseudomonas aeruginosa by measuring heat flow using isothermal calorimetry

AU - Beilharz, Katrin

AU - Kragh, Kasper Nørskov

AU - Fritz, Blaine

AU - Kirkegaard, Julius B.

AU - Tolker-Nielsen, Tim

AU - Bjarnsholt, Thomas

AU - Lichtenberg, Mads

N1 - Publisher Copyright: © 2023 The Authors

PY - 2023

Y1 - 2023

N2 - Here, we present a protocol for assessing metabolic activity of bacterial populations by measuring heat flow using isothermal calorimetry. We outline the steps for preparing the different growth models of Pseudomonas aeruginosa and performing continuous metabolic activity measurements in the calScreener. We detail simple principal component analysis to differentiate between metabolic states of different populations and probabilistic logistic classification to assess resemblance to wild-type bacteria. This protocol for fine-scale metabolic measurement can aid in understanding microbial physiology. For complete details on the use and execution of this protocol, please refer to Lichtenberg et al. (2022).1

AB - Here, we present a protocol for assessing metabolic activity of bacterial populations by measuring heat flow using isothermal calorimetry. We outline the steps for preparing the different growth models of Pseudomonas aeruginosa and performing continuous metabolic activity measurements in the calScreener. We detail simple principal component analysis to differentiate between metabolic states of different populations and probabilistic logistic classification to assess resemblance to wild-type bacteria. This protocol for fine-scale metabolic measurement can aid in understanding microbial physiology. For complete details on the use and execution of this protocol, please refer to Lichtenberg et al. (2022).1

KW - Cell-based Assays

KW - Metabolism

KW - Microbiology

U2 - 10.1016/j.xpro.2023.102269

DO - 10.1016/j.xpro.2023.102269

M3 - Journal article

C2 - 37133990

AN - SCOPUS:85153888219

VL - 4

JO - STAR Protocols

JF - STAR Protocols

SN - 2666-1667

IS - 2

M1 - 102269

ER -

ID: 346204389