Relation of intracellular signal levels and promoter activities in the gal regulon of escherichia coli

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Relation of intracellular signal levels and promoter activities in the gal regulon of escherichia coli. / Krishna, Sandeep; Orosz, László; Sneppen, Kim; Adhya, Sankar; Semsey, Szabolcs.

In: Journal of Molecular Biology, Vol. 391, No. 4, 2009, p. 671-678.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Krishna, S, Orosz, L, Sneppen, K, Adhya, S & Semsey, S 2009, 'Relation of intracellular signal levels and promoter activities in the gal regulon of escherichia coli', Journal of Molecular Biology, vol. 391, no. 4, pp. 671-678. https://doi.org/10.1016/j.jmb.2009.06.043

APA

Krishna, S., Orosz, L., Sneppen, K., Adhya, S., & Semsey, S. (2009). Relation of intracellular signal levels and promoter activities in the gal regulon of escherichia coli. Journal of Molecular Biology, 391(4), 671-678. https://doi.org/10.1016/j.jmb.2009.06.043

Vancouver

Krishna S, Orosz L, Sneppen K, Adhya S, Semsey S. Relation of intracellular signal levels and promoter activities in the gal regulon of escherichia coli. Journal of Molecular Biology. 2009;391(4):671-678. https://doi.org/10.1016/j.jmb.2009.06.043

Author

Krishna, Sandeep ; Orosz, László ; Sneppen, Kim ; Adhya, Sankar ; Semsey, Szabolcs. / Relation of intracellular signal levels and promoter activities in the gal regulon of escherichia coli. In: Journal of Molecular Biology. 2009 ; Vol. 391, No. 4. pp. 671-678.

Bibtex

@article{dac3eeb014a411df803f000ea68e967b,
title = "Relation of intracellular signal levels and promoter activities in the gal regulon of escherichia coli",
abstract = "Transcription of many genes is regulated by combinations of multiple signals. In Escherichia coli, combinatorial control is typical in the case of operons related to utilization of different sugars in the absence of glucose. To understand regulation of the transport and metabolic pathways in the galactose system, we measured activities of the six gal regulon promoters simultaneously, using an in vitro transcription system containing purified components. Input functions were computed on the basis of the experimental measurements. We observed four different shapes of input functions. From the results, we can conclude that the structure of the regulatory network is insufficient for the determination of signal integration. It is the actual structure of the promoter and regulatory region, the mechanism of transcription regulation, and the interplay between transcription factors that shape the input function to be suitable for adaptation.",
keywords = "Escherichia coli, Escherichia coli Proteins, Galactose, Gene Expression Regulation, Bacterial, Promoter Regions, Genetic, Regulon, Signal Transduction, Transcription Factors, Journal Article, Research Support, N.I.H., Intramural, Research Support, Non-U.S. Gov't",
author = "Sandeep Krishna and L{\'a}szl{\'o} Orosz and Kim Sneppen and Sankar Adhya and Szabolcs Semsey",
year = "2009",
doi = "10.1016/j.jmb.2009.06.043",
language = "English",
volume = "391",
pages = "671--678",
journal = "Journal of Molecular Biology",
issn = "0022-2836",
publisher = "Academic Press",
number = "4",

}

RIS

TY - JOUR

T1 - Relation of intracellular signal levels and promoter activities in the gal regulon of escherichia coli

AU - Krishna, Sandeep

AU - Orosz, László

AU - Sneppen, Kim

AU - Adhya, Sankar

AU - Semsey, Szabolcs

PY - 2009

Y1 - 2009

N2 - Transcription of many genes is regulated by combinations of multiple signals. In Escherichia coli, combinatorial control is typical in the case of operons related to utilization of different sugars in the absence of glucose. To understand regulation of the transport and metabolic pathways in the galactose system, we measured activities of the six gal regulon promoters simultaneously, using an in vitro transcription system containing purified components. Input functions were computed on the basis of the experimental measurements. We observed four different shapes of input functions. From the results, we can conclude that the structure of the regulatory network is insufficient for the determination of signal integration. It is the actual structure of the promoter and regulatory region, the mechanism of transcription regulation, and the interplay between transcription factors that shape the input function to be suitable for adaptation.

AB - Transcription of many genes is regulated by combinations of multiple signals. In Escherichia coli, combinatorial control is typical in the case of operons related to utilization of different sugars in the absence of glucose. To understand regulation of the transport and metabolic pathways in the galactose system, we measured activities of the six gal regulon promoters simultaneously, using an in vitro transcription system containing purified components. Input functions were computed on the basis of the experimental measurements. We observed four different shapes of input functions. From the results, we can conclude that the structure of the regulatory network is insufficient for the determination of signal integration. It is the actual structure of the promoter and regulatory region, the mechanism of transcription regulation, and the interplay between transcription factors that shape the input function to be suitable for adaptation.

KW - Escherichia coli

KW - Escherichia coli Proteins

KW - Galactose

KW - Gene Expression Regulation, Bacterial

KW - Promoter Regions, Genetic

KW - Regulon

KW - Signal Transduction

KW - Transcription Factors

KW - Journal Article

KW - Research Support, N.I.H., Intramural

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.jmb.2009.06.043

DO - 10.1016/j.jmb.2009.06.043

M3 - Journal article

C2 - 19559028

VL - 391

SP - 671

EP - 678

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 4

ER -

ID: 17496525