Thermoplasmonic nano-rupture of cells reveals Annexin V function in plasma membrane repair
Research output: Contribution to journal › Journal article › Research
Standard
Thermoplasmonic nano-rupture of cells reveals Annexin V function in plasma membrane repair. / Moreno-Pescador, Guillermo S.; Aswad, Dunya S.; Florentsen, Christoffer D.; Bahadori, Azra; Arastoo, Mohammad R.; Danielsen, Helena Maria D.; Heitmann, Anne Sofie B.; Boye, Theresa L.; Nylandsted, Jesper; Oddershede, Lene B.; Bendix, Poul Martin.
In: Nanoscale, Vol. 14, 2022, p. 7778–7787.Research output: Contribution to journal › Journal article › Research
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Thermoplasmonic nano-rupture of cells reveals Annexin V function in plasma membrane repair
AU - Moreno-Pescador, Guillermo S.
AU - Aswad, Dunya S.
AU - Florentsen, Christoffer D.
AU - Bahadori, Azra
AU - Arastoo, Mohammad R.
AU - Danielsen, Helena Maria D.
AU - Heitmann, Anne Sofie B.
AU - Boye, Theresa L.
AU - Nylandsted, Jesper
AU - Oddershede, Lene B.
AU - Bendix, Poul Martin
PY - 2022
Y1 - 2022
N2 - Maintaining the integrity of the cell plasma membrane (PM) is critical for the survival of cells. While an efficient PM repair machinery can aid survival of healthy cells by preventing influx of extracellular calcium, it can also constitute an obstacle in drug delivery and photothermal therapy. We show how nanoscopic holes can be created in a controlled fashion to the cell's plasma membrane, thus allowing identification of molecular components which have a pivotal role in PM repair. Cells are punctured by laser induced local heating of gold nanostructures at the cell surface which causes nano-ruptures in cellular PMs. Recruitment of annexin V near the hole is found to locally reshape the ruptured plasma membrane. Experiments using model membranes, containing recombinant annexin V, provide further biophysical insight into the ability of annexin V to reshape edges surrounding a membrane hole. The thermoplasmonic method provides a general strategy to monitor the response to nanoscopic injuries to the cell surface which offer new insight into how cells respond to photothermal treatment.
AB - Maintaining the integrity of the cell plasma membrane (PM) is critical for the survival of cells. While an efficient PM repair machinery can aid survival of healthy cells by preventing influx of extracellular calcium, it can also constitute an obstacle in drug delivery and photothermal therapy. We show how nanoscopic holes can be created in a controlled fashion to the cell's plasma membrane, thus allowing identification of molecular components which have a pivotal role in PM repair. Cells are punctured by laser induced local heating of gold nanostructures at the cell surface which causes nano-ruptures in cellular PMs. Recruitment of annexin V near the hole is found to locally reshape the ruptured plasma membrane. Experiments using model membranes, containing recombinant annexin V, provide further biophysical insight into the ability of annexin V to reshape edges surrounding a membrane hole. The thermoplasmonic method provides a general strategy to monitor the response to nanoscopic injuries to the cell surface which offer new insight into how cells respond to photothermal treatment.
U2 - 10.1039/D1NR08274D
DO - 10.1039/D1NR08274D
M3 - Journal article
VL - 14
SP - 7778
EP - 7787
JO - Nanoscale
JF - Nanoscale
SN - 2040-3364
ER -
ID: 284191900